MOBILISE develops a novel quality assured, container-based, mobile One Health laboratory solution, to provide the first BSL4 capacity to many European countries. It receives human/animal/environmental samples for molecular diagnostics, serology, microbiology, and host a One Health whole genome sequencing platform for pathogen discovery and epidemiological analysis. The development of a novel rapid diagnostic test is scheduled, customized to European BSL-3/4 pathogens, and machine readable from within the BSL-4 glovebox. A novel AI-based “Emergency Operating Centre and decision support system” software assists end-users in coordinating MOBILISE laboratory fleets across Europe and manage outbreaks in real-time. The laboratory is field-tested by National agencies and first-responders in Austria, Romania, Greece, and Africa to TRL-7. Through smart engineering a major novelty is the green laboratory design on an electric/hybrid truck platform, its energy-efficient workflows and equipment, and the use of solar and wind-energy – compliant with the European Green Deal.